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Thermoluminescence System TL 500

PSI Thermoluminescence Systems are designed to investigate structure of energetic levels in the Photosystem II. Light-induced charge separation in the Photosystem II reaction centers results in accumulation of radical pairs that store the absorbed light energy. Heating induces recombination of these radical pairs and it triggers light emission and formation of characteristic thermoluminescence glow curves. The shape and the peak position of the different thermoluminescence bands provide valuable information about the energetic stability of the respective radical pairs as well as about the functioning of the Photosystem II reaction centers. Interpretation of the obtained data requires a thorough understanding of the charge pairs responsible for generating different thermoluminescence bands.

High-temperature thermoluminescence appears as a result of accumulation of lipid peroxides and can be used as a simple and efficient tool to monitor oxidative stress in plants.
  • Different versions for measuring in broad temperature range from -90 to +175 °C
  • Variable high-intensity light source (typically 627 nm) used as a single turnover light for S state transition analysis
  • Variable long-duration actinic light source (typically 627 nm) for illumination at predefined temperatures
  • Sensitive photomultiplier for thermoluminescence glow peak detection
  • Protocol controlled electronic shutter preventing the photomultiplier from damage
  • Precisely linear heating process controlled by thermo-electric cooler regulation in the whole heating range
  • Ambient light protection enabling measurements in standard laboratory conditions
  • Additional air input for forced atmosphere inside the measuring chamber (in TL 500/HT only)
  • Liquid nitrogen tank with solenoid valve included in the TL 500/LT version
  • Thermoluminescence TL 500
  • Non-invasive monitoring of PSII electron transport
  • PSII adaptation and responses to abiotic and biotic stresses and structural modifications
  • Sensitive test of energetic imbalance in chloroplasts during various stress conditions
  • Redox reactions of PSII in thylakoid membrane
  • Interpretation of changes in the redox potentials of donors and acceptors on the basis of a shift in glow peak temperature
  • Oscillation pattern of thermoluminescence denoting the S state transition and its use for a titre of the Mn clusture of oxygen evolving complex
  • Thermoluminescence TL 500
  • Thermoluminescence TL 500/ST - standard version intended for investigations of Q, A, B1, B2, C, and AG glow curve peaks

    Standard Thermoluminescence Scheme
  • Thermoluminescence TL 500/HT - high-temperature version intended for investigations of glow curves peak measurements in the range up to +175°C (additional air input for forced atmosphere inside the measuring chamber included in the price)

    Standard Thermoluminescence Scheme
  • Thermoluminescence TL 500/LT - low-temperature version for temperature range from -90 °C can additionally monitor Z1 glow curve peak (liquid nitrogen tank included in the price)

    Standard Thermoluminescence Scheme
  • Typical samples are leaf segments, algal cells, thylakoids, or PSII preparations
  • Sample disc made of gold-plated copper has a diameter of 14 mm (in standard and low-temperature versions)
  • Sample disc for high-temperature version has a diameter of 22 mm (also made of gold-plated copper)
  • Sample Disc with a Leaf Segment Sample
    Sample Disc with a Leaf Segment Sample
  • FluorWin software for designing and saving experimental protocols
  • FluorWin Wizard for automated protocols scripting
  • Retrieval and export of experimental data to standard data processors
  • Data manipulation and visualization
  • Windows XP and higher compatible
  • Four Lights - Scope Measuring
    Typical Thermoluminescence Control Signals in Time Scale
  • Temperature Range:
    Standard version TL 500/ST: -25 ºC to +70 ºC
    Low-temperature version TL 500/LT: -90 ºC to +70 ºC
    High-temperature version TL 500 HT: -15 ºC to +175 ºC
  • Temperature Mode:
    Constant
    Linear change (0.1 ºC/sec - 2 ºC/sec; in the version TL 500/LT: 0.1 ºC/sec - 1 ºC/sec)
  • Overheating Protection:
    Yes
  • Minimum Sampling Period:
    100 ms
  • Control Regime:
    Manual (constant temperature)
    Protocol defined temperature profiles
  • Sample Disc:
    Made of gold-plated copper
    14 mm diameter in the standard and low-temperature version
    22 mm diameter in the high-temperature version
  • Typical Sample:
    Algal or cyanobacterial suspensions;
    Leaf segments
  • Light Source Characteristics:
    Saturating Pulse 627 nm; intensity up to 250,000 µmol(photon).m-2.s-1
    Actinic light 627 nm; intensity up to 1,500 µmol(photon).m-2.s-1
  • Detection Sensor:
    Photomultiplier with sensitivity software control
  • Spectral Response:
    300 nm - 900 nm
  • Switch-On Delay:
    100 ms
  • Ambient Light Protection:
    Provided
  • Control:
    Custom defined protocols with variable timing, special language and scripts
  • Communication:
    RS232/USB
  • Software:
    FluorWin 3.7
  • Electrical:
    90V - 240V
  • Warranty:
    1 year parts and labor
  • Nitrogen Tank
    Nitrogen Tank for TL 500/LT
  • DING S., JIANG R., LU Q. ET AL.(2016): Glutathione reductase 2 maintains the function of photosystem II in Arabidopsis under excess light. Biochimica et Biophysica Acta- Bioenergetics. Volume 1857, Issue 6, Pages 665–677. DOI: 10.1016/j.bbabio.2016.02.011
  • GAWRONSKI P., GORECKA M., BEDERSKA M. ET AL. (2013): Isochorismate synthase 1 is required for thylakoid organization, optimal plastoquinone redox status, and state transitions in Arabidopsis thaliana. Journal of Experimental Botany. Volume 64. Pages 3669-3679. DOI:10.1093/jxb/ert203
  • DING S., WEN X. AND LU C. (2013): Thermoluminescence (TL) Measurements in Tobacco Leaves. Bio-protocol, Volume 3
  • ALLAHVERDIYEVA Y., MAMEDOV F., HOLMSTROM M., ET AL. (2009): Comparison of the electron transport properties of the psbo1 and psbo2 mutants of Arabidopsis thaliana. BBA. Volume 1787. Pages 1230-1237. DOI:10.1016/j.bbabio.2009.05.013
  • LINTALA M., ALLAHVERDIYEVA Y., KANGASJÄRVI S., ET AL. (2009): Comparative analysis of leaf-type ferredoxin-NADP+ oxidoreductase isoforms in Arabidopsis thaliana. The Plant Journal, 57: 1103–1115. DOI:10.1111/j.1365-313X.2008.03753.x
  • STEGLICH C., BEHRENFELD M., KOBLIZEK M., ET AL. (2001): Nitrogen deprivation strongly affects Photosystem II but not phycoerythrin level in the divinyl-chlorophyll b-containing cyanobacterium Prochlorococcus marinus. Biochimica et Biophisica Acta. Volume 1503. Pages 341-349. DOI:10.1016/S0005-2728(00)00211-5

Pricing

  • Orders and Payments
  • Thermoluminescence System TL 500/ST
    24.990,- €
  • Thermoluminescence System TL 500/HT
    32.990,- €
  • Thermoluminescence System TL 500/LT
    34.990,- €

Thermoluminescence Versions

Thermoluminescence TL 500/ST
Standard version providing temperature control in the range of -25 °C to +70 °C.


Thermoluminescence TL 500/HT
High-temperature version providing temperature control in the range of -15 °C to +175 °C.


Thermoluminescence TL 500/LT
Low-temperature version providing temperature control in the range of -90 °C to +70 °C.

Download

Software
ThermoWin 3.6
OS: Win2000/XP
Language: English
Size: 1.4 MB


Documents
Thermoluminescence System TL 200 Manual
Type: PDF
Language: English
Size: 1.5 MB


Thermoluminescence Explain
Type: PDF
Language: English
Size: 342 KB


Thermoluminescence - List of References
Type: PDF
Language: English
Size: 1.1 MB


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