Closed GFPCam FC 800-C/1010-GFP is a GFP version of the Closed FluorCam and represents a highly innovative, robust and user-friendly world-wide used system for combined multispectral and kinetic fluorescence imaging. The basic system of the Closed FluorCam (CCD camera, four fixed LED panels) is supplemented by a fully motorized and software-controlled filter wheel and appropriate filter sets for detection and imaging of green fluorescent protein (GFP), red-shifted GFP (EGFP), or other variants of fluorescent proteins. The blue emitting and red fluorescent protein can be detected and imaged in absence of chlorophyll.
The GFPCam consists of a CCD camera, four to five fixed LED panels (4 + 1 additional, which is not included in the standard setup) and of a filter wheel equipped with up to 7 different emission filters. The LED panels provide uniform irradiance over sample area – suitable for imaging of small plants (such as Arabidopsis thaliana), detached leaves, mosses, lichens, plated algal colonies or algal suspensions, etc. The system is very compact and allows easy dark adaptation of an investigated sample
The Closed GFPCam generates images of fluorescence signal at any moment of the experiment and presents them using a false color scale. Full kinetic analysis is available. In all applications, the camera allows imaging of fluorescence transients that are induced by actinic light or by saturating flashes. The timing and amplitude of actinic irradiance are determined by user-defined protocols. The device configuration also provides user-friendly and quick switching between the GFP and chlorophyll fluorescence detection.
The GFPCam includes a high-performance PC and comprehensive software package comprising full system control, data acquisition and image processing. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences.
- Combined imaging of GFP and chlorophyll fluorescence
- Included high-resolution CCD camera TOMI-2: resolution 1360 x 1024 pixels, 20 images per second, video and snapshot operating modes. The camera is specifically intended for detection of weak signals demanding long integration times; the setup retains also most functionalities for chlorophyll fluorescence kinetics measurement
- Four light-upgraded LED panels (4 panels 470 nm OR 2 panels 447 nm + 2 panels 470 nm OR 2 panels 627 nm and 2 panels 470 nm)
- Additional UV, green, yellow, amber, orange or far-red light source (optional)
- 7 position filter wheel - motorized and software-controlled (included)
- Selectable shelf system for different plant sizes (included)
- Dark room for adaptation (included)
- Coming with a high-end computer and user-friendly software (included)
- Kautsky induction
- QA reoxidation (needs optional accessories)
- Fast fluorescence induction (OJIP) (needs optional accessories)
- Leaves, stems, seeds, roots, whole small plants
- Calluses, seedlings on petri plates
- Fruits and vegetables
- Cyanobacteria, green algae, bacteria
- Small animals
- LED panels 13 x 13 cm for uniform illumination over sample area
- Inner Dimension: 450 mm (W) x 450 mm (D) x 400 mm (H)
GFP Expression in Corn Kernels (GFPCam Captured)
Proteins of the GFP family demonstrate a great spectral diversity and they often have different excitation and emission spectra. Therefore it is important to use the most appropriate filter sets so as to obtain maximum sensitivity for FP viewing.
- Red-shifted variant ( EGFP)
The excitation peak is centered at about 488 nm and the emission peak is at 507 - 509 nm.
Suggested filters: short-pass 480 nm for excitation and 532/28 or 530/25 for emission.
- Original (wild type) GFP variant (wtGPF)
The major excitation peak is centered at about 395 - 397 nm and the emission peak is at 504 nm.
Suggested filters: short-pass 420 nm for excitation and 532/28 or 530/25 for emission.
- Blue-emitting variant (BFP)
The excitation peak at about 384 nm and the emission peak near 448 nm.
Suggested filters: short-pass 400 nm for excitation and 469/35 for emission.
- PSI LED panels used for excitation of most common fluorochromes
- List of most common fluorochromes and types of suitable excitation LED panels
- Automated experimental protocols via a Windows Wizard
- Multiple (automatically repeated) experiments
- Automated labeling of individual plants, or samples, within the field of view
- Kinetic analysis of data from all samples within the field of view
- Numerous image manipulation tools
- Imaging masks for 384-well plate, 96-well plate, Petri dish, etc.
- Barcode reader support
- Export to Excel
- Windows 2000, XP, Vista compatible*
Corn Kernels Captured by the Closed GFPCam:
- GFP Variant in the Upper Lines
- Control Kernels in the Bottom Lines
- Leal M.C. et al (2014): Marine Ecology (2014): 1-13, doi: 10.1111/maec.12164
- Orders and Payments
- Closed GFPCam FC 800-C/1010-GFP 22.350,- €
- Additional Far-Red Light Panel 1.990,- €
- Additional Ultraviolet Light Panel 2.990,- €
- Additional Single Color Light Panel 2.490,- €
- Additional Bi-Color Light Panel 2.790,- €
- QA-Reoxidation Module 11.000,- €
- PAR Absorptivity and NDVI Module 5.480,- €
- Multicolor Plant Module * 8.990,- €
- Fast Fluorescence Induction (OJIP) Module 6.900,- €
- Advanced Multiple Function for FluorCam Software 400,- €
- Advanced Module for Automatic and Programmable Image Acquisition and Data Management 5.990,- €
- Grid for Sample Holders 20,- €
- Barcode Reader with Supporting Software 910,- €
- Additional Protective Glasses * 19,- €
High-end option allowing determination of QA-reoxidation kinetics. QA-reoxidation is recorded in a pump-and-probe manner, i.e. using repetitive flashes and recording with variable delay after each flash.
PAR-Absorptivity and NDVI Module
High-end option for measuring PAR absorptivity and NDVI reflectance index. It includes 7-position filter wheel, light panel 13 x 13 cm (installed round the camera and mounted with far-red LEDs 740 nm + deep-red LEDs 660 nm) and a glass filter. PAR-measurement is based on reflectance of plants in near infrared region and in the visible red range of spectrum. NDVI is used as vegetative index for predicting photosynthetic activity.
IMPORTANT NOTES: To be used only with FC 800-C/1010, FC 800-C/1010-GPF or FC 800-O/1010. This module cannot be used with the Multicolor Plant Module in the FC 800-C/1010 or FC 800-C/1010-GPF.
Multicolor Plant Module
High-end option for measuring emission spectra in plants (according to Lichtenthaler and Bushmann). It includes 7-position filter wheel, UV LED light panel (installed round the camera and mounted with 4 UV LEDs 380 nm), four filters - BLUE (FF01-440/40), GREEN (FF02-520/28), RED (FF01-690/8), FAR-RED (FF01-747/33) - and a special software module.
IMPORTANT NOTES: To be used only in the FC 800-C/1010, FC 800-C/1010-GPF or FC 800-O/1010. This module cannot be used with the PAR-Absorptivity and NDVI Module in the FC 800-C/1010 or FC 800-C/1010-GPF.
Fast Fluorescence Induction (OJIP) Module
Electronic module for tracking fast fluorescence induction – OJIP curve. Includes a special electronic board and measuring protocol. The processes are recorded in multiple light exposures, each with a variable delay after the light is switched on.
Additional LED Light Panels
Additional LED light panel is mounted at the top part of the FluorCam System. It is located under the camera; the objective of the camera is inserted in the central opening of this panel. The panel widens the possibility of employing more excitation colors for special applications. It is equipped with 4 high-power LEDs in one-channel version, or 8 high-power LEDs in two-channel version. Available colours are: far-red (740 nm), deep-red (660 nm), ultra-violet (365 or 385 nm), green (523 nm), amber (590 nm). Other options available upon request.
Ultraviolet Panel (365 or 385 nm): Recommended use: (1) multicolor fluorescence - UV excited blue F440, green F520, red F680, and infra-red F740 (must be accompanied with the filter wheel and high-resolution CCD); (2) excitation source for wtGFP, DAPI (filter wheel and high-resolution CCD required). Optical power 1,5 W.
Blue Panel (470 nm): Recommended use: (1) excitation source for EGFP (must be accompanied with the filter wheel and high-resolution CCD); (2) studies on stomata function.
Green Panel (530 nm): Recommended use: excitation source for YFP (must be accompanied with the filter wheel and high-resolution CCD).
Far-Red Panel (740 nm) : Maximum total output: 1,200 mW (4 x 300 mW). Recommended use: determination of Fo' and PAR absorbance (use of filter wheel required).
PAR - NDVI Panel (660 nm + 740 nm): This panel is part of the PAR-Absorptivity and NDVI Module.
Grids for Sample Holders
Grids serve for firm and secure attachment of sample holders - Petri dishes of multi-well plates - to the desired spot on the FluorCam shelf or bottom. They are manufactured in several versions: 90 mm, 100 mm or 137 mm Petri dishes, for 120 mm square Petri dish or for multi-well plates.
Additional Protective Glasses
Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge.
Advanced Multiple Function for FluorCam Software
Software upgrade enabling multiple scripting functions for automatic initiation and measurement of scheduled FluorCam protocols and for automatic data storage. Advanced multiple function feature allows to define time of protocol initiation with user-defined protocol, apply user defined mask for feature segmentation, automatically subtract the background based on the mask definition and analyze the measured data. The are stored as TAR files with time and date description. Useful for circadian cycle study.
Advanced Module for Automatic and Programmable Image Acquisition and Data Management
Comprehensive SW package for system control, data acquisition, image analysis and data base configuration. User friendly graphical interface PlantScreenTM Scheduler is designed to control hardware system components actions and to design experiments with an extremely high level of flexibility. Scheduling assistant implements calendar function and is designed for visualization, planning and management of the individual experiments. Complex protocols can be easily designed including dark/light adaptation schemes and executed based on user defined actions. All acquired imaging data are stored in an SQL database, processed and available for inspection and further analysis in range of seconds after recording via user-friendly graphical interface. It is an excellent tools for rapid and easy data browsing, grouping, analysis, user-defined data reprocessing and export. Key features are: straightforward access to analyzed parameters and acquired images over the time series of measurements, graphical display of trends for individual or multiple parameters, basic statistical analysis, visualization of time series image data for selected parameters and easy export function for numeric and image-based data for user defined sets of experiments. Multiple clients can be connected to the database, with different privileges assigned based on a built-in authentication mechanism.
Barcode Reader with Supporting Software
Barcode reader with FluorCam supporting software included. Used for fully automatic bar code search.
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OS: Win XP/Vista/Seven (32bit)
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Open FluorCam FC 800-O is a highly modular instrument with flexible geometry. The LED panels and the light source generating saturating flashes can be arranged at various angles and distances from the sample. Also, the position of the camera relative to the sample is adjustable.
Closed FC 800-C
Closed FluorCam FC 800-C represents a highly innovative, world-wide used system of multispectral kinetic fluorescence imaging. It consists of a CCD camera and four fixed LED panels. The LED panels provide uniform irradiance over samples up to 13 x 13 cm – suitable for small plants, detached leaves, algal dilutions, etc. The system allows dark adaptation.
Closed GFPCam FC 800-C/1010GFP
Closed GFPCam FC 800-C/1010GFP serves for various applications using green fluorescent proteins in molecular and cellular biology. The device is equipped with a motorized, software-controlled filter wheel, which provides quick and simple transition between the GFP and chlorophyl fluorescence detection and imaging.
Handy FC 1000-H
Handy FluorCam FC 1000-H is a portable system designed for kinetically resolved fluorescence imaging of leaves and small plants both in a field and laboratory. The whole instrument can be carried in a convenient bag over the shoulder and can run on batteries.
Handy GFPCam FC 1000-H/GFP
Handy GFPCam FC 1000-H/GFP is a modified version of the popular Handy FluorCam. Its unique construction allows both GFP imaging and chlorophyll fluorescence imaging. It can be favorably used for lab and field experiments.
Handy - Leaf Chamber FC 1000-LC
Leaf Chamber Fluorcam FC 1000-LC is a unique system that allows you to conduct chlorophyll fluorescence imaging on a leaf maintained in a leaf chamber, while measuring leaf gas exchange at the same time. The Fluorcam unit can be attached to leaf chambers provided by most manufacturers of leaf gas exchange equipment.